ABSTRACT
Aim: Effect of gamma irradiation on genomic disorder in sesame are scanty. The present study was undertaken to evaluate the mutagenic effects of gamma rays on different parameters in two popular cultivars of sesame, Roma and Tilottama. Methodology: Seeds of these two cultivars were irradiated with five doses of gamma rays: (250, 300, 350, 400, and 450 Gy) at BARC, Trombay and were sown (along with the un-irradiated control) during March 2015 in a split plot design with 3 replications keeping row to row and plant to plant distance at 30 cm and 10 cm, respectively to determine mutagen sensitivity with regard to pollen fertility (%), germination (%) and seedling height (cm), root-shoot length (cm), plant survival (%) at maturity in M1 generation. To study mutability, four to five capsules from each M1 plants in all the treatments were collected separately to give rise the M2 generation. Individual plant progeny rows were sown in M2 during March 2016. ID50 was determined by probit analysis for germination, shoot-root length and plant survival. Since the dose requirement for pollen fertility is very high, ID30 was calculated instead of ID50 value. Results: It was observed that with increasing doses of gamma rays, the response of all characters decreased significantly and followed a linear relationship in both varieties. The root system was more profound to gamma rays than the shoot. Chlorophyll mutations showed independent response to different doses of gamma rays as they occurred in random. The mutability of genotype Roma induced with different doses of gamma rays was higher than that of Tilottama. Interpretation: Due to saturation in the mutational actions, response of characters decreased with increase in gamma ray doses but magnitudes of effect differed between genotypes. The cultivar Tilottama was found to be more sensitive than Roma.
ABSTRACT
Objective To study the mutability of ultra sodium pyrosulfite intake on ultrastructure changes and spermatogonium mice testis.Methods Forty male Kunming mice were used.Experimental group had been exposed to ultra sodium pyrosulfite by fed for 10 days,and sodium pyrosulfite′s contaminated dose were 1% and 1‰.Mice were killed at 11~(th) day,and ultrastructure changes were observed under transmission electron microscopy(TEM) and the tests of sister chromosome exchanges(SCE) were made.Mmutation of ultra sodium pyrosulfite on spermatogonium of mice testis was judged.Results Compared with control group,there was a significant increase of SCE ratio in spermatogonium of testis in experimental groups(P
ABSTRACT
Analyses the results of 14 kinds of drugs in mutational tests by grey cluster analysis were reported. A more rational mathematical model for quantitative evaluating the results was established. According to the value of grey relationship grades, this drugs were classified into 4 groups by "Net-making" cluster: strong mutagen, medium mutagen, weak mutagen and non-mutagen. The mode} could be calculated simply and its calculating results had a high correspondency with special qualitative analysis. In addition the function of CHL test, MN test and Ames test were evaluated by grey superiority analysis.